B.A. 1968, Haverford College, Haverford, PA
Ph.D. 1974, Indiana University, Bloomington, IN
Postdoctoral Fellow, Harvard Medical School, Boston, MA
Postdoctoral Fellow, Worcester Foundation for Experimental Biology, Shrewsbury, MA
Analysis of the human genome suggests the presence of about 22,000 protein coding genes, far fewer than originally expected. However, other observations indicate a much greater level of genetic complexity than this number suggests. For example, most pre-mRNAs are alternatively spliced, with some genes giving rise to dozens and even hundreds of mRNAs and proteins. In addition, most of the genome does not code for protein or pre-mRNA at all. Rather, it is transcribed to yield non-coding RNAs, many of which are highly regulated but of unknown function. My interests include the role of protein-RNA interactions in alternative splicing, mechanisms of antisense regulation and the evolution of alternatively spliced genes and non-coding RNAs.
Munroe, S.H., Morales C.H., Duyck T.H. and Waters, P.D. 2015. Evolution of the antisense overlap between genes for thyroid hormone receptor and Rev-erbα and characterization of an exonic G-Rich element that regulates splicing of TRα2 mRNA. PLoS One 10(9):e0137893. http://www.ncbi.nlm.nih.gov/pubmed/26368571
Dorweiler, J.E., Ni, T., Zhu, J., Munroe, S.H. and Anderson, J.T. 2014. Certain adenylated non-coding RNAs, including 5' leader sequences of primary microRNA transcripts, accumulate in mouse cells following depletion of the RNA helicase MTR4. PLoS One 9(6):e99430. http://www.ncbi.nlm.nih.gov/pubmed/24926684
Munroe, S.H. and Zhu, J. 2006. Overlapping transcripts, double-stranded RNA and antisense regulation: A genomic perspective. Cell. Mol. Life Sci. 63, 2102-18. http://www.ncbi.nlm.nih.gov/pubmed/16847578