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Animal Care Procedures and the Animal Health Program

The following is a description of the general procedures used to provide care for laboratory animals at Marquette University. Since every experiment has slightly different environmental and technical requirements, these guidelines remain sufficiently flexible to provide care individualized to the needs of each investigator.

The investigator must order animals through purchasing. At this time the IACUC Secretary will check for a current protocol and the approved number of animals to be used. The Animal Care Facility Supervisor will also be consulted to determine space availability.

Care of Laboratory Rodents

Upon arrival rats and mice are uncrated and placed into clean cages segregated by strain and sex. Each cage is identified with a cage card stating the investigator, sex, age, source of the animals and the date received. Unless otherwise instructed, the animals are given a standard diet and water ad libitum. The animals are checked thoroughly for signs of disease or parasites. For the first week, the new animals are kept isolated from the resident population to prevent cross contamination.


Daily Duties:

Rodents are closely observed on a daily basis for signs of illness or distress. Ill animals are reported to the Supervisor. Animals which are found dead should be bagged and placed into a freezer unless other special instructions have been given by the investigator. The cages are checked to assure an adequate supply of dry bedding, food and water. The room is dusted, swept and mopped.

Once Per Week:

The animals receive clean water. This may occur more frequently if necessary.

Two times/week:

Rats are moved to clean cages.

New cage tops are used.

Once/week:

Mice are moved to clean cages.

Sinks and other equipment are cleaned.

Water bottles are washed.

Other husbandry and housekeeping tasks are performed at the request of the investigator.

Care of Laboratory Rabbits

When Rabbits have been delivered, the federal record of transfer is completed. Noted is the type of acquisition, dealer's license number, seller, USDA license number, and the number and condition of the animals. The animals are put in clean cages and provided with fresh food and water ad libitum. Each animal is assigned an identification number and an identification card is prepared for each animal. Recorded on the card is information such as seller/breeder, date received, weight, name of the investigator, and date and explanation of any procedures performed on the animals while they are alive. A copy of the card is attached to the cage and another copy is filed as is the federal record of transfer. A separate page for each animal is filed as is the federal record of transfer. A separate page for each animal is filled out in the Rabbit Health Record to record the results of the initial and weekly health checks. A health check should include weight and general observations such as sneezing or nasal discharge, soiled fur, sores on ears or back, or excessively long nails. If an animal is ill or any distress or injury is noted, the Supervisor should examine the rabbit immediately. If the problem is simple (i.e. long nails or dirty ears) it can be attended to quickly. If the condition is more serious, the investigator or the veterinarian is called.

Daily Care:

Hosing the fecal collection pans.

Rinsing and refilling water bottles.

Checking feeders for signs of food dust or mold and filling them with fresh, clean food.

The room is swept, including air vents.

The floor is mopped daily.

The sink and any recently used equipment are washed following use.

Bi-Weekly Care:

Rabbits are moved to clean cages.

Weekly Care:

Water bottles are changed.

Monthly:

Rabbits are given a health check (including weighing) each month and the information is recorded in the Rabbit Health Record.



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